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KMID : 0364820100460020162
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Korean Journal of Microbiology
2010 Volume.46 No. 2 p.162 ~ p.168
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Analysis of Sequence Diversity of mcyA Gene Involved in Microcystin Synthesis in Korean Reservoirs
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Oh Kyoung-Hee
Han Ah-Won
Cho Young-Cheol
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Abstract
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The sequence diversity of mcyA gene involved in synthesis of microcystins was analyzed in Microcystis spp. isolated from the Korean reservoirs and in the environmental samples taken from the Daechung, Chungju, Yongdam, Soyang, and Euam Reservoirs at the cyanobacterial blooming season. It was estimated that the sequences of mcyA gene in the isolated Microcystis spp. were much conserved when compared with those in GenBank database. A few kinds of clones were dominant in the investigated environmental samples, occupying 87 to 100% of total clones. No mcyA sequences originated from Anabaena spp. or Planktothrix spp. was found. These results indicated that microcystins are produced
mainly by Microcystis spp. and the sequences of mcyA genes are much conserved in the investigated Korean reservoirs.
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KEYWORD
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Korean reservoirs, mcy gene, mcyA, Microcystis, microcystins
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